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BDA can form adducts with protein (including histones) after exposure to furan in vivo , especially with lysine, and form adducts with free nucleosides (in particular with deoxyribose guanosine, ‐cytosine and –adenine) and DNA adducts in purified DNA and bacteria

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Precipitates, formed in the heated reaction mixture, were cleared by centrifugation at 13,000 × g for 3 min